Protein Purification Reagents and Kits
Genscript Corporation Anti-DYKDDDDK IP Resin
GenScript Anti-DYKDDDDK IP Resin is designed for quick and efficient immunoprecipitation of DYKDDDDK-tagged protein or DYKDDDDK-tagged protein-target protein complex The resin is an agarose beads coupled to Mouse anti-DYKDDDDK monoclonal antibody (clone 6H8E4) The antibody recognizes N-terminal internal and C-terminal fusion protein containing DYKDDDDK tag It is supplied as a suspension in 50 % glycerol containing 10 mM sodium phosphate 150 mM sodium chloride pH 7 4 0 02 % (w/v) sodium azide (PBS/A)
Waters Corporation pH Concentrate, 10X, pH 10.2
This 10X buffer concentrate B is specifically optimized for use in creating a pH 5.0 to pH 10.2 gradient, essential for monoclonal antibody (mAb) charge variant separations. It is designed to be used alongside BioResolve SCX columns and features a pH of 10.2.
- Optimized for creating a pH 5.0 to pH 10.2 gradient.
- Aids in monoclonal antibody (mAb) charge variant separations.
- Designed for use with BioResolve SCX columns.
- Features a pH of 10.2.
New England Biolabs, Inc. Amylose Resin High Flow 15 ml
Amylose Resin High Flow is a cross-linked affinity matrix used for the isolation of proteins fused to maltose-binding protein (MBP). This rigid matrix can be used in automated chromatography systems.
LifeSensors Tandem Ubiquitin Binding Entities (M1 Linear) Biotin 50µg
LifeSensors has developed TUBE (Tandem Ubiquitin Binding Entity) technology for the detection, characterization and isolation of polyubiquitylated proteins from cells and tissue extracts. TUBEs capitalize on the linkage of multiple ubiquitin interacting motifs (UIMs) to generate reagents with a high affinity for polyubiquitin. TUBEs have 100 to 1000-fold higher affinity for polyubiquitin chains compared to monomer ubiquitin binding domains (UBDs). TUBEs both stabilize and bind to ubiquitylated proteins, serving as an indispensable tool for ubiquitologists. Our M1 (linear) TUBE has high selectivity for M1-linked polyubiquitin over the more common K48- and K63-linked polyubiquitin chains, making it a powerful tool for studying the biological consequences of this ubiquitin linkage type. This product is protected by one or more US or Foreign patents
Biochain Institute Inc Membrane Protein - Human Adult Normal Tissue: Thyroid, 0.1 mg/PK
Tissue membrane protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis. The protein is stored in a buffer with protease inhibitor cocktail.
Hampton Research HR2-130/Crystal Screen HT - 1 ml, Deep Well block format
HR2-130/Crystal Screen HT - 1 ml, Deep Well block format. Please note items from this supplier are non-returnable.
Biotium Indo-1, AM Ester, 10x100 ug
Indo-1, AM ester is membrane-permeant and thus can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127, a mild detergent, is often used as a dispersing agent to facilitate cell loading. Indo-1/AM itself does not bind Ca2 , but it is readily hydrolyzed to Indo-1 by endogenous esterases once it enters cells. Properties: Light yellow solid soluble in DMSO; Store at -20°C and protect from light, especially in solution; C47H51N3O22; MW: 1010; [112926-02-0]
Active Motif Nuclear Complex Co-IP Kit
Active Motif's Nuclear Complex Co-IP Kit simplifies co-immunoprecipitation of nuclear protein complexes by providing high-quality reagents for both nuclear extract preparation and immunoprecipitation. The kit's extraction process maintains protein complexes contained in the nucleus, specifically those previously bound to DNA, while the stringency of the Co-IP reagents can be easily adjusted so you can better study protein/protein interactions of varying strength.
Revvity Health Sciences Inc LabChip High pI Charge Variant Analysis LabChip
LabChip High pI Charge Variant Analysis LabChip
Hampton Research HR4-957/Mounted CryoLoop - 20 micron, 0.2 - 0.3 mm - 25 pack
HR4-957/Mounted CryoLoop - 20 micron, 0.2 - 0.3 mm - 25 pack. Please note items from this supplier are non-returnable.
Hampton Research Detergent Screen, 0.25 mL, Deep Well block format
Manipulate sample-sample and sample-solvent interactions, including non-specific aggregation due to hydrophobic interactions, to alter sample solubility, or obtain or improve crystalline samples.
Invent Biotechnologies Inc Minute™ Nuclear Proteasome Enrichment Kit - Spin Column-Based (20 preps)
The proteasome is a protein degradation system that requires metabolic energy and polymerization of ubiquitin that is different from lysosome-based protein degradation. The 26S proteasome is made up of two subcomplexes (a 20S proteasome and one or two19S regulatory particles). The proteasomes have been found mainly localized in cytosol but also detected in nucleus. To overcome these shortcomings, we have developed spin-column-based kits for enrichment of proteasome from nuclei (Cytosolic Proteasome Enrichment Kit is also available under Cat # PT-040). The protocol is simple and rapid with high yield. The gentle protocol maintains the association of proteasomes with ubiquitin and other proteins and is useful for the studies of proteasome structure and function. The highly enriched proteasomes can also be used as a starting material for affinity purifications.
ATHENA ES 100 mM Triton X-100
Detergents have been shown to aid in the protein refolding process both of native and recombinant proteins. Detergents are usually used as a step in the protein refolding process, often in conjunction with cyclodextrins. The detergent binds to the protein in the dilution stage to form a detergent-protein complex which prevents aggregation of the protein. A binding agent, such as cyclodextrin, can then be used to strip away the detergent from the protein, allowing it to properly refold. Athena’s Detergent Screening Kit provides researchers with 6 common detergents (10mL each) for determining the optimum detergent for a given protein refolding scenario. Individual detergents can also be purchased in 100mL quantities.
Revvity Health Sciences Inc PhenoVue Multi Organelle Staining Kit 1x384
PhenoVue Multi Organelle Staining Kit 1x384